![]() Scale bar, 70 µm, in D, also refers to E. Grey arrows indicate Vangl2 aggregate orientation. (D,E) GFP and Vangl2 immunostaining of NPs grafted with DBL (D, n=7) or VE (E, n=3) at stage 12/12.5. Yellow boxes approximate the regions shown in (D) and (E). ![]() (B,C) Brightfield images of neurula embryos grafted with DBL (B) and VE (C) (green) to the anterior NP. (A) Schematic of dorsal blastopore lip (DBL) and ventral fragment (VE) grafting experiment. Published by The Company of Biologists Ltd.ĭorsal blastopore lip grafts exhibit PCP-inducing activity. Xenopus embryos Dorsal blastopore lip Induction Neuroectoderm PCP Prickle3 Signaling Vangl2. These observations suggest that neuroectodermal PCP is not instructed by a preexisting molecular gradient but induced by a signal from the dorsal blastopore lip. The PCP cue did not depend on the orientation of the graft and was distinct from neural inducers. Tissue transplantations indicated that PCP is triggered in the neural plate by a planar cue from the dorsal blastopore lip. By imaging Vangl2 and Prickle3, we show that PCP is progressively acquired in the neural plate and requires a signal from the posterior region of the embryo. ![]() Here we investigate the Xenopus neural plate, a tissue that has been previously shown to exhibit PCP. Although the segregation of PCP components to opposite cell borders is believed to play a critical role in this pathway, whether PCP derives from egg polarity or preexistent long-range gradient, or forms in response to a localized cue, remains a challenging question. Coordinated polarization of cells in the tissue plane, known as planar cell polarity (PCP), is associated with a signaling pathway critical for the control of morphogenetic processes. ![]()
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